OS optimization is very important when you want to use VMware Horizon and deliver virtual desktop machines to your user. As you may know, there is a fling (VMware OS Optimization Tool) that made by VMware engineers and you can use it to optimize your OS automatically.
VMware has published a guide for this tool and you can download it fro this link: VMware Windows Operating System Optimization Tool Guide
I suggest that read the above guide before do any action on your template and deploy machine from that.